GSM7903093: WT_0h-3; Schizosaccharomyces pombe; RNA-Seq - SRA

SRX22541091: GSM7903093: WT_0h-3; Schizosaccharomyces pombe; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 22.5M spots, 2.3G bases, 752.9Mb downloads

External Id: GSM7903093_r1

Submitted by: Stewart Shuman, Molecular Biology, Memorial Sloan Kettering Cancer Center

Study: Factors governing the transcriptome changes and chronological lifespan of fission yeast during phosphate starvation II

PRJNA1040914 • SRP472248 • All experiments • All runs

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This RNA-Seq analysis compares gene expression of the wild-type fission yeast (Schizosaccharomyces pombe) strain at various times after transfer to defined phosphate-replete ePMGT(+PO4) medium (2, 4, 8 HR) contrasting to the WT fission yeast S. pombe cells grown in phosphate-replete YES medium (0HR). Overall design: 3 test samples and 1 control sample was processed (each sample has 3 replicate experiments)

Sample: WT_0h-3

SAMN38271844 • SRS19550675 • All experiments • All runs

Organism: Schizosaccharomyces pombe

Library:

Name: GSM7903093

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: hot acid phenol RNA purification protocol RNA-seq libraries were prepared from polyA+ RNA using the Illumina TruSeq stranded mRNA Kit according to the manufacturers protocol. Indexed libraries were normalized and pooled for paired-end sequencing performed using an Illumina NovaSeq 6000 instrument at the Weill Cornell Medical College Genome Core Facility (in New York). Stranded polyA+ RNA Seq

Runs: 1 run, 22.5M spots, 2.3G bases, 752.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR26845603	22,489,064	2.3G	752.9Mb	2024-01-28

ID:: 30536128

SRA

Sequence Read Archive

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